This study compares a wound dressing based on bacterial cellulose/collagen (BC/COL) hydrogel in rat dorsum with commercial collagenase ointment and untreated wound. The hydrogel was characterized by Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), Fourier transformed - Infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). According to the In vivo test and macroscopic evaluation, BC/COL hydrogel showed a better repair of wounds and promoted statistically significant differences of tissue repair between treatments on the 7th day after surgery. Better quality, quantity and orientation evaluation of collagen fibers (p=0.0001) were observed in the BC/COL hydrogel and collagenase ointment groups in relation to the control group. The BC/COL hydrogel promoted better wound healing than collagenase and the control group, therefore, it can be considered a potential wound dressing for skin regeneration.

Objetivou-se, neste trabalho, obter biomembranas de colágeno tratadas em solução alcalina por 72 horas (GE), a partir de centros tendinosos diafragmáticos de equinos e comparar sua biocompatibilidade com membranas conservadas em solução de glicerina a 98% (GG) e membranas não tratadas (GC). As membranas foram implantadas na fáscia interna do músculo reto do abdome de equinos e retiradas, juntamente com os tecidos adjacentes, aos sete, 63 e 126 dias pós-operatórios para a preparação de lâminas histológicas. O estudo histomorfométrico das lâminas revelou processo inflamatório mais intenso para os implantes GG e CC e cicatrização mais rápida para os implantes GE. Concluiu-se que as biomembranas colagênicas tratadas em solução alcalina são mais biocompatíveis do que biomembranas conservadas em glicerina 98%.

The objective of this research was obtain collagen biomembranes treated in alkaline solution for 72 hours (GE) from tendineous diaphragmatic center of equines and compare its biocompatibility with membranes preserved in a glycerin solution 98% (GG) and membranes do not treated (GC). The membranes were implanted in the internal fascia of recto abdominis muscle of equines and removed, with adjacent tissues, seven, 63 and 126 days postoperative for the preparation of histological slides. The histomorphometric study revealed more intense inflammatory process to GG and CC implants and faster healing for GE implants. It was concluded that the collagen biomembranes treated in alkaline solution is more biocompatible than biomembranes preserved in 98% glycerin.